Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Positive ControlRat brain

Application

ELISA (see comments)r>Immunoblotting (1:1000)r>Frozen Sections (1:5000)r>Free-Floating Sections (1:5000)r>Paraffin Sections (not recommended)

General description

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D₁ receptor protein.

This Anti-Dopamine D₁ Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D₁ Receptor.

Recognizes the dopamine D₁ receptor in rat brain. Does not cross-react with other dopamine receptors.

Immunogen

Human

a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Warning

Toxicity: Standard Handling (A)