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Analysis Note

ControlHeLa acid extracts, HEK 293 nuclear extracts.

Application

Use Anti-HP1γ Antibody, clone 42s2 (Mouse Monoclonal Antibody) validated in ChIP, IHC, WB to detect HP1γ also known as Heterochromatin protein 1 homolog gamma.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.

Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated

Immunogen

GST fusion protein corresponding to residues 17-173 of human HP1γ protein. Clone 42s2.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to license agreement restrictions, this product cannot be purchased for resale.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1 in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.

Protein G Purified

Format: Purified

Quality

Routinely evaluated by western blot in acid-extracted HeLa cell lysates (Catalog #13-112).

Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).

Specificity

This antibody recognizes HP1γ protein, Mr ~25 kDa.

Reactivity with other species has not been determined.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Target description

25 kDa