s6501-1kg

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. It is recommended for use in molecular biology applications at 25-50µg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L.

Biochem/physiol Actions

Mode of Action: Streptomycin acts by inhibiting prokaryote protein synthesis by binding to the S12 protein of the 30S ribosomal subunit, thus preventing the transition from imitation complex to chain-elongating ribosome. This causes miscoding or inhibits initiation.Mode of Resistance: A mutation in rpsL, a gene for S12 ribosomal protein, prevents binding of streptomycin to the ribosome. An aminoglycoside phosphotransferase also inactivates streptomycin.Antimicrobial spectrum: Streptomycin acts against gram-negative and gram-positive bacteria.

Caution

Solutions can be stored at 2-8°C for up to a month or at -20°C for more extended periods. At 37°C, solutions are stable for three days.

General description

Chemical structure: aminoglycoside

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place

Packaging

5, 25, 50, 100 g in poly bottle

1 kg in poly bottle

Preparation Note

Stock solutions should be prepared directly in the vial with sterile water at concentrations between 20-25 mg/mL.

antibiotic activity spectrum	Gram-positive bacteria, Gram-negative bacteria, mycobacteria
biological source	Streptomyces griseus
color	white to off-white
form	powder
InChI key	QTENRWWVYAAPBI-YZTFXSNBSA-N
InChI	1S/2C21H39N7O12.3H2O4S/c21-5-21(36,4-30)16(40-17-9(26-2)13(34)10(31)6(3-29)38-17)18(37-5)39-15-8(28-20(24)25)11(32)7(27-19(22)23)12(33)14(15)35;31-5(2,3)4/h24-18,26,29,31-36H,3H2,1-2H3,(H4,22,23,27)(H4,24,25,28);3(H2,1,2,3,4)/t2*5-,6-,7-,8+,9-,10-,11-,12+,13-,14-,15-,16-,17-,18-,21+;;;/m00.../s1
mode of action	protein synthesis \| interferes
mol wt	calculated mol wt 1457.38 ((C₂₁H₃₉N₇O₁₂)₂ ⋅3H₂O₄S)
potency	≥720 I.U. per mg
Quality Level	200
SMILES string	OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@H]2[C@@H](O[C@@H](C)[C@]2(O)C=O)O[C@@H]3[C@@H](O)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]3NC(N)=N.CN[C@H]4[C@H](O)[C@@H](O)[C@H](CO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)[C@]5(O)C=O)O[C@@H]6[C@@H](O)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]6NC(N)=N
storage condition	(Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
storage temp.	2-8°C