Microwell sandwich ELISAs, used for the quantitative analysis of low levels of residues in food products, ingredients, clean in place (CIP) rinses and environmental swabs in a 96 well format. The quantity of protein in the sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

These ELISA kits utilise a sandwich ELISA. The target protein present in the sample react with the specific antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antibodies conjugated with horseradish peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with the previously bound target protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The colour development from this enzymatic reaction varies directly with the concentration of target protein in the sample tested; thus, the absorbance, at 450nm, is a measure of the concentration of the target protein in the test sample. The quantity of protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

• Can accommodate environmental swab, CIP rinse water and food samples
• A universal extraction protocol streamlines testing for multiple analytes
• Environmental collection swabs sold separately