Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

The biological activity is measured in a cell proliferation assay using the murine mast cell line, MC/9.

Application

Interleukin-10 (IL-10) from rat has been used to study the anti-allodynic effect of IL-10 in rat model.

Biochem/physiol Actions

Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1⁺ (CD5⁺⁾, and normal B cells. In human, the cellular sources of IL-10 consist of CD4⁺ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.

Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Physical form

Lyophilized from a 0.2 µm filtered in 20 mM Tris, 0.1 M sodium chloride containing 50 µg of bovine serum albumin per 1 µg of cytokine.