Not available outside of the UK & Ireland.

Components

Pan-Enterovirus Blend (Catalog No. 3360)One 5 mL dropper vial containing a blend of two mouse monoclonal antibodies which recognize all enteroviruses including echoviruses, coxsackie A and B viruses, polio 1,2, and 3, and enteroviruses 70 and 71, protein stabilizer and 0.1% sodium azide (preservative). The Pan-Enterovirus Blend may cross-react with hepatitis A, reovirus 3, astrovirus and rhinovirus.

Disclaimer

For in vitro Diagnostic UseCE Mark

General description

Diagnostics Kit

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Materials Required But Not Provided:
⋅ Acetone, reagent grade; stored in glass

⋅ Distilled water or deionized water

⋅ Sodium hypochlorite solution, 0.05% (1:100 dilution of household bleach)

⋅ Sterile shell vials with 12 mm coverslips, or culture tubes containing monolayer of cell line appropriate for growth of enteroviruses (RMK, MRC-5, etc)

⋅ Tissue culture media such as RPMI or Eagle′s Minimum Essential Medium (EMEM) with fetal bovine serum (FBS) and antibiotics, or equivalent

⋅ Viral transport medium which is non-inhibitory to enteroviruses

⋅ 0.1N NaOH

⋅ 0.1N HCl

⋅ Microscope slides, non-fluorescing

⋅ No. 1 cover slips

⋅ Negative and positive control slides

⋅ Mounting Fluid (Catalog No. 5013)

⋅ Anti-Mouse IgG:FITC Conjugate (Catalog No. 5008)

⋅ Normal Mouse Antibody (Catalog No. 5014)

⋅ PBS, 0.01 M, pH 7.1 to 7.4 with 0.085% NaCl and 0.1% sodium azide, (Catalog No. 5087)

⋅ 100X Wash Solution Concentrate (5% Tween 20 / 2% Sodium Azide Solution) (optional) (Catalog No. 5037)

⋅ Blocking solution, such as 1% casein or Difco Bacto Skim Milk with, 0.05% Tween 20, 0.02% sodium azide.

⋅ Centrifuge capable of 700-950 x g with biohazard buckets and adapters for shell vials

⋅ Fluorescence microscope with appropriate filter combination for FITC (excitation peak = 490 nm, emission peak = 520 nm) with 100x, 200x, 400x magnification (dry objective)

⋅ Forceps

⋅ Humid chamber

⋅ Incubator, 37 * 1*C

⋅ Syringe filter, 0.45 micron

⋅ Ultrasonic water bath

⋅ Vortex mixer or sonicator

Format: Purified

Storage and Stability

When stored at 2-8°C, the Pan-Enterovirus Blend is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures. Discard any remaining reagent after the expiration date.

Warning and Precautions:

⋅ Incubation times or temperatures other than those specified may give erroneous results. Any change must be validated by the user.

* The performance of the Light Diagnostics Pan-Enterovirus Blend has not been determined on direct specimens.

⋅ The monoclonal antibodies present in the Pan-Enterovirus Blend may show some background in certain cell lines, such as HEp-2 cells or some monkey cell lines.

⋅ Slides prepared too early (﹤25% CPE) or too late (>95% CPE) can be difficult to read and can lead to false negative results.

⋅ Sodium azide, present in the reagent, may react with lead and copper plumbing to form potentially explosive metal azides. Upon disposing of solutions that contain sodium azide, flush plumbing with a large volume of water to prevent build-up.

* Handle all specimens and materials coming in contact with them as potentially infectious materials. Decontaminate with 0.05% sodium hypochlorite prior to disposal.

* Avoid contact with Evans blue, if present in any reagent, as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

* Do not mouth pipette reagents.

⋅ Do not allow shell vials or slides to dry at any time during the staining procedure.

⋅ Pooling or alteration of any reagent may cause erroneous results.

⋅ Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation.

⋅ Mounting Fluid (Catalog No. 5013) contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water.