illustra ProbeQuant G-50 Micro Columns

Code: 28903408 D2-84

  • ProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for the preparation of DNA probes, separating them from unincorporated labelled nucleotides

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  • ProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for the preparation of DNA probes, separating them from unincorporated labelled nucleotides
  • For rapid purification of labelled DNA (≥ 20 bases) from unincorporated labelled nucleotides in a volume of 25 to 50 μL using spin-column chromatography
  • Prepacked with Sephadex G-50 DNA Grade and pre-equilibrated in STE containing 0.15% Kathon CG/ICP Biocide for convenience
  • Ready to use and requires less than 4 minutes from sample application to collection of purified product
  • Designed for use in a microcentrifuge
  • Tested in assays: nickase, single and double-stranded exonuclease and RNase
  • ProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides, in addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation
  • The columns are designed for use in a microcentrifuge, come prepacked with Sephadex G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use, they can accommodate samples ranging in volume from 25 to 50µL
  • The resin used in these columns, Sephadex G-50 DNA Grade, can be used in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides
  • MicroSpin columns packed with Sephadex G-50 DNA Grade are ideal for the rapid purification of DNA for use in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides
  • Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established size exclusion chromatography resin for desalting and buffer exchange of biomolecules >30000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length
  • Alternatively, Sephadex G-25 or Sephadex G-100 can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length
  • Sephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA
  • All these Sephadex types are therefore suitable for the purification of oligonucleotides or small DNA fragments following synthesis or a labelling reaction

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