Superdex 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.

• Enhanced performance: improved resolution and runtime compared to predecessor Superdex 200
• Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity
• High flow rates: rigid beads give excellent pressure/flow properties

Superdex 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex Increase columns deliver higher resolution purification in shorter run times.

Superdex 200 Increase has a selectivity curve optimiSed for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations.

The outstanding resolving power of this resin facilitates protein characteriSation, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.

Superdex 200 Increase is optimiSed to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.

This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.