FTA technology consists of two distinct chemistries, both of which have the ability to lyse cells on contact, denature proteins, and protect DNA from degradation. FTA chemistry contains chemical denaturants and a free radical scavenger. With FTA, the DNA remains tightly bound while proteins and inhibitors are washed from the matrix. There are three options for DNA analysis with FTA cards: direct amplification, standard amplification with rinsing (“punch-in” method), and extraction of DNA from the card.
QIAcard FTA indicating cards are designed for collection, stabilisation, processing, transport, and archiving of buccal nucleic acid samples. The cards include a pink indicating dye that turns to white when a clear sample such as buccal cells or saliva is applied. Each sample area of a card can be processed separately. This versatility of handling supports manual or automated processing and incorporation into current workflows.
- Easy transfer and detection of colorless samples
- Improves process efficiency by enabling direct amplification for STR analysis
- Stabilisation and protection of nucleic acids at room temperature
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