Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Candida parapsilosis is a fungal species of the Saccharomycetaceae (yeast) family and is now one of the most common candidiasis species after C.albicans. C.parapsilosis is found abundantly in nature and is part of the natural flora of humans and mammals. It is also easily isolated from soil, seawater and plants. C.parapsilosis cells are oval, round or cylindrical shapes, it does not form true hyphae and

exists in yeast or pseudohyphal form. Candida parapsilosis has a lower virulence compared to C.albicans and has a lower mortality

and morbidity in neonates and adults. Common virulence factors between the two species include adherence to epithelial and endothelial cells, proteinase production, pseudohypha formation, phospholipase production and phenotypic switching. The lower virulence of C.

parapsilosis is due to its inability to form hyphae.

Individuals that Candida parapsilosis infection poses the biggest risk to includes neonates and patients in intensive care units. Infections are mostly associated with hyperalimentation units, prosthetic devices (e.g. valves) and indwelling catheters. The pathogenicity of C.

parapsilosis is limited by intact integument however it can grow in total parenteral nutrition and is able to form biofilms on catheters and other implanted devices – this can lead to wide raging infections including thrush, fungemia, endocarditis, endophthalmitis and arthritis. Fast and accurate detection of an C.parapsilosis by real time PCR could be greatly beneficial.

The Primerdesign genesig Kit for Candida parapsilosis (C.parapsilosis) genomes is designed for the in vitro quantification of C.parapsilosis genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water