Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Blue tongue virus (BTV) is an infectious, non-contagious, arthropodborne viral disease of ruminants such as sheep, cattle, deer, goats and camels. The virus is a member of the genus Orbivirus in the family Reoviridae. There are 24 serotypes of BTV that have been identified worldwide but they do not exist together in any single geographical region. They are closely related to the the epizootic haemorrhagic disease group. The virus is 86nm in diameter and has a linear double stranded RNA genome of approximately 19.2 kb in length. It is split into 10 segments that encode seven structural proteins (VP1 to VP7) and three non-structural proteins (NS1 to NS3). The genome is enclosed in a two layered capsid comprising the 470S core particle and an outer capsid protein layer. The outer capsid is shed on cell entry and further core uncoating reveals a 390S subcore that lacks the VP7 structural protein.

The Primerdesign genesig Kit for Bluetongue Virus (BTV) genomes is designed for the in vitro quantification of BTV genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water