Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

The widespread use of antibiotics has caused shifts in bacterial development to overcome the existing mechanisms of combating bacterial infections. Antibiotics such as penicillin and erythromycin, which used to have a high efficacy against many bacterial species and strains, have become less effective, due to the increased resistance of many bacterial strains. Antibiotic resistance is of particular concern, not only in human medicine but also in veterinary and agricultural medicine. The main cause for resistance development is effectively the selection and preferential growth of resistant bacteria, coupled with inhibition of susceptible strains from antibiotic use. Additionally, the survival of bacteria often results from an inheritable resistance developed via mutation, although the growth of antibiotic resistance also occurs through horizontal gene transfer. This is more likely to happen in locations of frequent antibiotic use. Essentially, horizontal gene transfer is another method of passing genetic information aside from traditional vertical transfer. (I.E. reproduction).

Genes that are responsible for antibiotic resistance in one species of bacteria can be transferred to another species of bacteria through various mechanisms subsequently equipping the recipient with antibiotic resistant genes. Use of these genesig kits allows for the screening of specific mutations encoding for enzymes which enable resistance. By screening a sample it is then possible to determine any possible resistance and then deducing a preferred format of treatment or other course of action.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water