Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Borrelia burgdorferi is a Gram negative aerobic bacterium which is a causative agent of Lyme disease. It is considered to be the most common tick-borne bacterial disease in the world. They belong to the spirochete class, genus Borrelia and have a single stranded, linear DNA genome of approximately 910 kb in length. The genome contains approximately 853 genes coding for basic functions and has several accompanying plasmids. It is a helical bacterium with an inner and outer membrane as well as a flexible cell wall. The cell is normally only 1µm wide but due to its cylindrical shape can be 10-25µm long. It is structurally distinct as the flagella are located inside the periplasm between the inner and outer cell membranes. These allow the cell to travel through the tissue of its infected host or vector, causing it to be highly invasive.

The Primerdesign genesig Kit for Borrelia burgdorferi (B.burgdorferi) genomes is designed for the in vitro quantification of B. burgdorferi genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water