Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Avian orthoreovirus (also known as Avian Reovirus) is an orthoreovirus from the Reoviridae family. Avian orthoreoviruses (ARVs) are responsible for considerable economic losses in broiler chickens. The full coding region has been determined for two ARV strains associated with tenosynovitis. The complete genome is 23,494 bp in size and included 12 ORFs. Avian orthoreoviruses are ubiquitous among poultry flocks and have been reported globally. It is transmitted mainly horizontally via the faecal: oral route. Vertical transmission does occur but at low rates.

Virus can be isolated from the respiratory, enteric and reproductive tracts or from tendons, plasma and blood cells of recently killed birds. Its site of primary replication is the epithelia of the small intestine. Infection induces formation of syncytia, degeneration and holes in the monolayer. Presence can be confirmed by qPCR.

The Primerdesign genesig Kit for Avian orthoreovirus (A.orthoreovirus) genomes is designed for the in vitro quantification of A.orthoreovirus genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water