Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Aspergillus niger is a haploid filamentous fungi that is widely exploited by the fermentation industry in the production of enzymes and organic acids, in particular citric acid. The fungi is ubiquitous and has been found in a wide variety of environments including soil, plant debri, dust and paint. A.niger particularly favours warmer climates in the wider environment and stored foods. It’s characteristic black pores protect the fungus from sunlight and UV irradiation, A.niger is often isolated from sun-dried vine fruits. A.niger is capable of causing human disease, however it is significantly less likely than other Aspergillus species. Aspergillosis is a lung disease caused by aspergillus infection, this occurs more frequently in horticultural workers than the general population and is often caused by inhalation of Aspergillus spores found in peat dust . A.niger is a common cause of fungal ear infections.

Assays are designed to specifically detect the query target(s) at the >95% homology and identity level in sillico and to prevent detection of any off-target sequences (unless specified).

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water