Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Clostridium perfringens is an anaerobic, spore-forming, non-motile, Gram positive, rod shaped bacterium of the genus Clostridium. It has a circular DNA genome of approximately 3Mb containing 2660 protein coding regions, and also carries genes on plasmids. It is widely

distributed in the environment and foods and forms part of the normal gut flora in both man and animals. C. perfringens persists in soil, sediments and areas subject to human or animal faecal pollution. In the UK and the USA it is the third most common cause of food poisoning, often being associated with large scale catering and is largely linked to C. perfringens type A.

C. perfringens produces at least 16 virulence factors, including 12 toxins. Strains of C. perfringens are classified as 5 biotypes A – E depending on the differential production of four major (i.e. lethal) exotoxins (alpha (phospholipase A), beta, epsilon and iota) which give the

cytotoxic and necrotic activity associated with infection. Clostridial haemolysins and extracellular enzymes such as proteases, lipases, collagenase and hyaluronidase contribute to the invasive process and cytotoxicity. In addition, strains of C. perfringens may also produce a number of other toxins, including: neuraminidase and enterotoxin (Cpe).

Our kit for All pathogenic C.perfringens strains (C.perfringens_cpa) genomes is designed for the in vitro quantification of C.perfringens strains that have the cpa toxin gene conferring the ability to cause infection. The kit is designed to have the broadest detection profile possible whilst remaining specific to the C.perfringens_cpa target gene. The primers and probe sequences in this kit have 100% homology with over 95% of cpa toxin sequences in the NCBI database based on a comprehensive bioinformatics analysis.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water