Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Bovine viral diarrhoea virus (BVDV) is a contagious disease of domestic and wild ruminants. It is a single stranded RNA virus of the genus Pestivirus, family Flaviviridae and is closely related to classical swine fever and ovine Border disease viruses. BVDV occurs in two forms: noncytopathogenic and cytopathogenic. There are two antigenically distinct genotypes (types 1 and 2), and virus isolates within these groups exhibit considerable biological and antigenic diversity. BVDV-1 and BVDV-2 have subtle immunological differences. The genome of BVDV-1 is 12.57 kb long while that of the BVDV-2 is 12.26 kb long. Border disease virus affects lambs and is caused by pestivirus infection of the foetus in early pregnancy, the disease has been observed in most sheep-rearing regions of world and there are currently seven recognised genotypes. The infection causes low birth weight and viability, tremor, poor conformation and more hair on the coat of the animal at birth. Lambs which survive are viremic and the virus is present in their excretions. Other farmed animals including goats, pigs and cattle are also susceptible to border disease infection.

The Primerdesign genesig Kit for Bovine viral diarrhoea virus and Border Disease (BVDV & BDV) genomes is designed for the in vitro quantification of BVDV & BDV genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water