Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Anaplasma platys (A.platys) is a gram-negative, nonmotile parasitic bacterium present in the genus Anaplasma. Species within this genus cause Anaplasmosis, a tickborne disease that infects red blood cells. Anaplasmosis in its more severe form is most commonly associated with Anaplasma phagocytophilum. A.platys causes a less potent type of Anaplasmosis: thrombocytotropic anaplasmosis/infectious canine cyclic thrombocytopenia (ICCT) that leads to a periodic decrease in platelets, and is transmitted by the brown dog tick. Clinical signs of canine anaplasmosis include lethargy, high fever and tend to mirror those seen in dogs with Lyme disease. Anaplasmosis is a zoonotic disease, known to infect humans and a range of animals. Strict tick control measures should be applied if a dog is diagnosed with anaplasmosis to prevent spread of the disease.

Serological testing via ELISA or IFA for example is prone to cross-reactivity and tends to lack in sensitivity. Real time PCR is a more sensitive and specific method for accurate identification of A. platys infection.

The Primerdesign genesig Kit for Anaplasma platys (Path-A.platys) genomes is designed for the in vitro quantification of Path-A.platys genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water