Not available outside of the UK & Ireland.

Application

Ubiquitin human can be used as a test compound for studying the isolation and characterization of rice E3-ubiquitin ligase and the role of OsHOS1 gene in the modulation of the cold stress response.

Ubiquitin human has been used: as a substrate in in vitro ubiquitination assaysas substrate in ADP-ribosylation and ubiquitylation assaysto supplement the 64 ng of endogenous ubiquitin in fresh blood for experiment grouping in order to study its potential clinical impact on cancer prognosis

N-Terminal histidine-tagged ubiquitin can replace native ubiquitin in formation of poly-ubiquitin—protein conjugates. The histidine tag enables separation and enrichment of protein conjugates on a Ni(II) column and detection of conjugates in western blot by anti-histidine-tag antibodies.

Biochem/physiol Actions

Ubiquitin plays a key role in normal eukaryotic cell function. It participates in the development and function of the immune system. This immunophilin is also known to participate in immune homeostasis. The ubiquitin peptide fragment (U50-59) is capable of blocking cellular and humoral immunity.

Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.

General description

Ubiquitin, a globular protein is made of 76 amino acids and has lysine residues on its surface. It has a molecular weight of 8565 Da.

Packaging

1 mg in glass bottle

Preparation Note

Ubiquitin human can dissolved in 0.02 M Tris-HCl at a concentration of 1.00 - 1.10 mg/ml to yield a clear to slightly hazy, colorless solution.