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Biochem/physiol Actions

The expression of ICAM1 (intercellular adhesion molecule 1) is strongly increased by IL-1 (interleukin) and interferon (IFN)-γ. It is expressed on lymphoid cells where it is required for phorbol ester-induced aggregation. It participates in LFA-1 (lymphocyte function-associated antigen)-dependent NK (natural killer) cell stimulation through its attachment to actin cytoskeleton through α-actinin or ezrin. It also acts as the receptor for rhinovirus, a common cold virus and Plasmodium falciparum. Soluble ICAM-1 levels are elevated in pregnant women with gestational diabetes mellitus (GDM), which is an outcome of endothelial cell activation and dysfunction. Meta-analysis of case-control studies show that rs1799969 polymorphism in ICAM-1 gene is linked with increased susceptibility to cancer.

General description

ICAM1 (intercellular adhesion molecule 1) was initially identified as a ligand for the β2 integrin lymphocyte function-associated antigen (LFA)-1. It is a glycoprotein with a 90,000-110,000Mr molecular weight, and shows wide range of tissue expression, including tissue macrophages and dendritic cells. It also shows expression on non-hematopoietic cells such as vascular endothelial cells, epithelial cells of thymus and mucosa, and dermal fibroblasts. It is a member of the ICAM family, which also include ICAM-2, -3, -4 and -5.Recombinant human ICAM-1 is a 49.5 kDa glycoprotein comprising the extracellular domain (453 amino acid residues) of ICAM-1. Monomeric glycosylated ICAM-1 migrates at an apparent molecular weight of approximately 72.0-80.0 kDa by SDS-PAGE analysis under reducing conditions.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

QTSVSPSKVI LPRGGSVLVT CSTSCDQPKL LGIETPLPKK ELLLPGNNRK VYELSNVQED SQPMCYSNCP DGQSTAKTFL TVYWTPERVE LAPLPSWQPV GKNLTLRCQV EGGAPRANLT VVLLRGEKEL KREPAVGEPA EVTTTVLVRR DHHGANFSCR TELDLRPQGL ELFENTSAPY QLQTFVLPAT PPQLVSPRVL EVDTQGTVVC SLDGLFPVSE AQVHLALGDQ RLNPTVTYGN DSFSAKASVS VTAEDEGTQR LTCAVILGNQ SQETLQTVTI YSFPAPNVIL TKPEVSEGTE VTVKCEAHPR AKVTLNGVPA QPLGPRAQLL LKATPEDNGR SFSCSATLEV AGQLIHKNQT RELRVLYGPR LDERDCPGNW TWPENSQQTP MCQAWGNPLP ELKCLKDGTF PLPIGESVTV TRDLEGTYLC RARSTQGEVT RKVTVNVLSP RYE