Beta D-1 (47 aa) human,;, recombinant, expressed in E. coli, >=98% (SDS-PAGE), >=98% (HPLC), cell culture tested

Code: srp3012-20ug D2-231

Not available outside of the UK & Ireland.

Biochem/physiol Actions

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune s...


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$417.24 20UG

Not available outside of the UK & Ireland.

Biochem/physiol Actions

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. Recombinant human BD-1 is a 5.0 kDa protein containing 47 amino acid residues.

General description

Chemical structure: peptide

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36a.a.), a propeptide region. b-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Recombinant human BD-1 is a 5.0 kDa protein containing 47 amino acid residues.

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 4.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK

antibiotic activity spectrumfungi, viruses, mycobacteria
assay≥98% (SDS-PAGE), ≥98% (HPLC)
biological sourcehuman
colorwhite to off-white
formlyophilized
Gene Informationhuman ... DEFB1(1672)
impurities﹤0.1 EU/µg endotoxin, tested
mode of actioncell membrane | interferes
mol wt5.0 kDa
packagingpkg of 20 µg
recombinantexpressed in E. coli
shipped inwet ice
storage temp.−20°C
technique(s)cell culture | mammalian: suitable
UniProt accession no.P60022
This product has met the following criteria: