Not available outside of the UK & Ireland.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

UbcH13 (GenBank Accession No. NM_003348), amino acids 2-end, with N-terminal His-tag, MW = 17.6 kDa, expressed in an E. coli cell expression system.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.