Zebrafish fibroblast growth factor 2 (FGF-2/bFGF/basic FGF) has been used extensively to support the maintenance and proliferation of human and mouse induced pluripotent (iPSC) and embryonic stem cells (ESC), used in the original Ludwig et al feeder-free culture of embryonic stem cells protocols 1-3.

High purity 17 kDa FGF-2 / bFGF protein, animal origin-free (AOF) and carrier-protein free (CF).

• High purity zebrafish Danio rerio FGF-2/bFGF (residues 2-154, Uniprot: B3DGE3) as described in Ludwig et al
• >98%, by SDS-PAGE quantitative densitometry
• 17 kDa
• Expressed in E. coli
• Animal origin-free (AOF) and carrier protein-free.
• Manufactured in our Cambridge, UK laboratories
• Lyophilised from Tris/NaCl/CyS/mannitol
• Resuspend in water at >100µg/mL, prepare single use aliquots, add carrier protein if desired, store frozen at -20°C (short-term) or -80°C (long-term)