Not available outside of the UK & Ireland.

Application

Tris buffered saline with TWEEN 20; pH 8 is used as a washing buffer in assays such as ELISA and western blotting.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

TWEEN is a registered trademark of Croda International PLC

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.