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Biochem/physiol Actions

Insulin-activated Mg²⁺-dependent and Ca²⁺-stimulated mitochondrial protein phosphatase specific for the pyruvate dehydrogenase complex.

Pyruvate dehydrogenase phosphatase catalytic subunit 1 (PDP1) catalyzes a Mg²⁺,-Ca²⁺ mediated dephosphorylation and activation of E1. PDP1 plays a crucial role in the regulation of pyruvate dehydrogenase complex (PDC) activity.

General description

Pyruvate dehydrogenase phosphatase (PDP) (EC 3.1.3.43) contains two genetically and biochemically different isoforms, PDP1 and PDP2. PDP1 is widely distributed and is composed of two subunits PDP1c, a catalytic subunit and PDP1r, a flavoprotein with a bound FAD.

Physical form

Solution in 50 µl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 50% glycerol.

Unit Definition

One unit will releases 1 nmol of inorganic phosphate from ³²P-labeled pyruvate dehydrogenase complex per min at 30 °C, pH 7.0.