Not available outside of the UK & Ireland.

Application

Wee1, active from rat may be used to study apoptotic signaling.

Biochem/physiol Actions

Wee1 is a protein tyrosine kinase that phosphorylates Cdc2 on Tyr15 and hence inactivates the CDK1 and CDK2, which facilitates in the negative regulation of entry of cells into mitosis. Increased level of wee1 stimulates Human Immunodeficiency Virus type 1 (HIV-1) Vpr induced cell cycle arrest at G2/M transition followed by apoptosis.

Protein inhibitor of Cdk2 kinase

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% Glycerol

Unit Definition

One unit of Wee 1 is defined as the amount of Wee 1 which inhibits 90% of the activity of 10 units of Cdk2