Not available outside of the UK & Ireland.

General description

MLK3 or Mixed-Lineage Kinase 3 is a mitogen-activated protein kinase kinase kinase capable of preferentially activating MAPK8/JNK kinase and functions as a positive regulator of JNK signaling pathway. MLK3 can directly phosphorylate and activate IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. MLK3 is a signal-integrating kinase with conventional MAP3K catalytic activity and additional noncatalytic functions that contribute to RAF/ERK signalling. MLK3 is a component of the BRAF/RAF1 complex and is required for integrity of the complex and for activation of ERK by the complex.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles