Not available outside of the UK & Ireland.

Biochem/physiol Actions

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apoptosis is achieved through binding to two death-signaling receptors, death receptor 4 and 5 (DR4 and 5). These receptors belong to the tumor necrosis factor receptor (TNFR) superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which activates the cell’;s apoptotic machinery.

General description

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a cytotoxic protein. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN