Not available outside of the UK & Ireland.

Application

4EBP1 Active human has been used as a substrate for in vitro kinase assay.

Biochem/physiol Actions

E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is involved in protein synthesis, cell growth and survival. In unphosphorylated form, it suppresses the translation initiation of capped mRNAs. On the other hand, in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eIF4E (eukaryotic translation initiation factor 4E), thereby resulting in cap-dependent translation. E1F4EBP1 is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, it interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21.

General description

The gene E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is mapped to human chromosome 8p11-p12. It belongs to the translation repressor protein family.Human E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1)(GenBank Accession No. NM_004095), full length with N-terminal His tag, MW = 13.6kDa, expressed in Escherichia coli.

Physical form

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 50% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.