Not available outside of the UK & Ireland.

Application

PTPN1 has been used for dephosphorylation of PP2A (protein phosphatase 2A).

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Biochem/physiol Actions

The protein protein tyrosine phosphatase-1B (PTP1B) catalyzes the dephosphorylation of tyrosine residues of the activated insulin receptor and the insulin receptor substrate 1, thereby downregulating the insulin signaling cascade. It dephosphorylates JAK2 and STAT3 and inhibits leptin signaling. Polymorphism of this gene in mice has shown to be associated with type 2 diabetes and obesity. PTP1B dephosphorylates the epidermal growth factor receptor. It acts as a modulator of insulin-like growth factor receptor (IGFR) and MET-mediated signaling responses. The protein may be useful as a drug target in type 2 diabetes and obesity. It has been shown to enhance tumor progression in various cancers like that of the breast, colon and prostate. It acts a tumor suppressor in other cancers (lymphomas and esophageal cancers).

General description

The gene PTPN1 (protein tyrosine phosphatase-1B) is mapped to human chromosome 20q13.13 and encodes a member of the protein tyrosine phosphatase (PTP) family. The open reading frame of 1305 bp encodes a protein of 435 amino acids. It is localized at the cytoplasmic side of the endoplasmic reticulum.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.