Not available outside of the UK & Ireland.

General description

Human SUV39H1 (GenBank Accession No. NM_003173), full length (amino acids 2-end), with N-terminal GST tag, MW = 74 kDa, expressed in Baculovirus infected Sf9 cell expression system.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.