Not available outside of the UK & Ireland.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Complex of: Human EZH1, GenBank Accession No. NM_001991, amino acids 2-end, with N-terminal His tag, MW = 86 kDa. Human EED, NM_003797, amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag. RbAp48 and AEBP2.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.