Sonic hedgehog protein (Shh) is a member of the Hedgehog family with an essential role in embryonic development, tissue regeneration, and tumorigenesis. Shh induces the cell fate and patterning of neural progenitors in ventral domains at various levels in the forebrain, midbrain, hindbrain, and spinal cord. It has many applications in the neural stem cell field where it plays a significant role in differentiating human-induced pluripotent stem cells (iPSC) towards motor neurons and interneurons. Also, it induces the patterning of organoids and embryos in culture.

Recombinant Shh protein has a molecular weight of 19.8kDa. This protein is animal origin-free (AOF), carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Sonic hedgehog protein is suitable for the culture of reproducible and high-quality neurons and organoids.

• High purity sonic hedgehog protein (Uniprot: Q15465)
• >98%, by SDS-PAGE quantitative densitometry
• 19.8 kDa (monomer)
• Expressed in E. coli
• Animal origin-free (AOF) and carrier protein-free
• Manufactured in Cambridge, UK
• Lyophilised from PBS
• Resuspend in water at >100µg/mL, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C (short-term) or -80°C (long-term)