Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.

Qk032 is a 17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal origin-free and carrier-protein free.

• High purity bioactive domain of human R-spondin 3 protein (Uniprot: Q9BXY4)
• >98%, by SDS-PAGE quantitative densitometry
• 17 kDa
• Expressed in E. coli
• Animal origin-free (AOF) and carrier protein-free
• Manufactured in our Cambridge, UK laboratories
• Lyophilised from acetonitrile, TFA
• Resuspend in 10mM HCl at >100µg/mL (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C