pro1086

OVERVIEW
RANGE
SUSTAINABILITY

R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signalling in many organoid culture systems including intestinal and tumour (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.

Recombinant human R-spondin 1 protein (RSPO1) protein is the bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1. Those are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture.

This protein has a molecular weight of 13 kDa and is animal origin-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk006 is suitable to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.

High purity recombinant human R-spondin 1 protein (Uniprot: Q2MKA7)
>98%, by SDS-PAGE quantitative densitometry
13 kDa
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10mM HCl at >100µg/mL (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

This product has met the following criteria to qualify for the following awards:

	Sustainable Raw Material	Designed to reduce the use of raw materials
	Sustainable Product Manufacturing	Measures were in place during the manufacture of this product to reduce energy consumption Measures were in place during the manufacture of this product to generate less waste Measures were in place during the manufacture of this product to reduce water usage Aligns with the principles of green chemistry
	Sustainable Distribution & Packaging	Manufactured in the UK or Ireland Country of origin has an Environmental Performance Index (EPI) greater than 60 Plastic free packaging Product reduces need for packaging
	Sustainable Use	Product contributes to a reduced energy consumption Product contributes to a reduced water consumption Product reduces or replaces the need for consumables Built to last – minimum 5-year warranty, and spare part/servicing available
	Sustainable Disposal	A feature of this product enhances its biodegradability