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Biochem/physiol Actions

Lambda protein phosphatase has been used to dephosphorylate HeLa cell extracts.

Can be used to release phosphate groups from serine, threonine or tyrosine residues in proteins. Also active on phosphorylated histidine residues.

General description

Lambda protein phosphatase is a recombinant protein expressed in Escherichia coli. It requires Mn²⁺ or Ni²⁺ as activators.

Unit Definition

One unit will hydrolyze 1 nanomole of p-nitrophenyl phosphate per min at pH 7.5 at 30 deg C.