Not available outside of the UK & Ireland.

Analysis Note

Protein determined by biuret.

Application

Phospholipase A2 has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation.

Biochem/physiol Actions

It has a high catalytic activity on aggregated substrates compared to monomeric substrates.

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

General description

Phospholipase A2 is a small disulfide-rich protein having 124 residues. It is a calcium-dependent enzyme.

Packaging

1, 5, 10 mg in glass bottle

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 5.5

Unit Definition

One unit will hydrolyze 1.0 µmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.