Not available outside of the UK & Ireland.

Analysis Note

The biological activity of recombinant mouse OSM was measured by its ability to stimulate ³H-thymidine incorporation in quiescent NIH/3T3 cells.

Application

Oncostatin M from mouse has been used in the preparation of culture medium for primary culture of mouse fetal hepatoblasts (MFHs).

Biochem/physiol Actions

Oncostatin M, LIF, G-CSF, IL-6, and ciliary neurotrophic factor (CNTF) are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components. Oncostatin M is a growth-regulating cytokine, affecting a number of tumor and normal cells. This material was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. It acts synergistically with TGF β1 to inhibit the proliferation of tumor cells like A375 melanoma cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. Oncostatin M is secreted by macrophages and activated T lymphocytes.

Physical form

Lyophilized from a 0.2 µm filtered solution in phosphate buffered saline containing 1.25 mg bovine serum albumin.