SODIUM DODECYL SULFATE

Code: l3771-10kg D2-231

Not available outside of the UK & Ireland.

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Application

Sodium dodecyl sulfate has been used: in chromatin immu...


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Your Price
$2,842.00 10KG

Not available outside of the UK & Ireland.

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Application

Sodium dodecyl sulfate has been used: in chromatin immunoprecipitation in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) as a component of radioimmunoprecipitation assay buffer for immunoblotting

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Anionic detergent

General description

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Packaging

25, 100, 500 g in poly bottle

1 kg in poly bottle

absorption≤0.1 at 260 at 3%, ≤0.1 at 280 at 3%
aggregation number62
anion tracesphosphate (PO43-): ≤10 ppm, chloride (Cl-): ≤500 ppm
assay≥98.5% (GC)
cation tracesheavy metals (as Pb): ≤10 ppm
CMC7-10 mM (20-25°C)
descriptionanionic
foreign activityDNase, RNase, none detected
gradefor molecular biology
HLB40
InChI keyDBMJMQXJHONAFJ-UHFFFAOYSA-M
InChI1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
mol wtmicellar 18,000
mp204-207 °C (lit.)
product lineBioReagent
Quality Level200
SMILES string[Na+].CCCCCCCCCCCCOS([O-])(=O)=O
solubilitywater: soluble
suitabilitysuitable for electrophoresis
technique(s)electrophoresis: suitable
transition tempcloud point >100 °C
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Cas Number151-21-3
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