Not available outside of the UK & Ireland.

Application

Immunoblotting (see comments)

Components

5X Re-Probe solution (125 ml)

General description

This Western-Re-Probe™ Reagent is validated for use in Immunoblotting.

Western-Re-Probe™ Reagent is specifically formulated to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity. The removal of antibodies also removes corresponding chemiluminescence or radioactive signals from the blots. Stripped blots can be probed with new probes.

Designed to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sufficient volume to strip 25-30 standard blots.r>r>ADVANTAGESr>-Immunoblotting analyzed repeatedly with multiple probesr>-Saving on hard-to-get reagents and samplesr>-Correction and re-analysis of unsatisfactory Western Blotsr>-Saving time spent in gel re-running and transfer processesr>r>PROTOCOLr>1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.r>2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.r>3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween™-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.r>4- Block the membrane and perform immunodetection.r>r>Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.r>r>PROTOCOL SUMMARYr>1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shakingr>2. Wash the membrane 3 times in PBST or TBST bufferr>3. Block membrane and perform immunodetection

Packaging

100 ml in Plastic ampoule

Physical form

Supplied as a 5X solution.

Warning

Toxicity: Standard Handling (A)