Not available outside of the UK & Ireland.

Application

The GlycoProfile^™ IV Kit utilizes trifluoromethanesulfonic acid (TFMS) in a deglycosylation system that completely removes all N- and O-linked glycans while preserving the protein/polypeptide structure. TFMS hydrolysis of glycoproteins results in minimal protein degradation while the released glycans are destroyed. For high molecular weight or complex, non-mammalian glycoproteins, an optional scavenger species is included. Complete deglycosylation of glycoproteins takes place in as little as 30 minutes.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Quantity

The kit contains sufficient reagents for 10 reactions with a sample size between 1-2 mg of a typical glycoprotein. An RNase B glycoprotein standard is included.