Not available outside of the UK & Ireland.
Biochem/physiol Actions
Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.
Packaging
Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.
Preparation Note
Expressed in glycosidase-free hosts.
Unit Definition
One unit will hydrolyze 1 µmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.
This product has met the following criteria: