Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-P, lyophilized powder (contains approx. 50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Not available outside of the UK & Ireland.

Analysis Note

Potency and solubility expressed in mg lectin/ml

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:for the stimulation of cluster of differentiation (CD4+) T cellsfor the activation of primary peripheral blood lymphocytesfor the stimulation of peripheral blood mononuclear cells

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

Sold on the basis of lectin content