Not available outside of the UK & Ireland.
Application
Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
General description
Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.Method of purification - Protein A
Immunogen
FLAG; peptide sequence DYKDDDDK
Legal Information
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes
Antibody is not calcium dependent.
Physical form
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)
Preparation Note
Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.
This product has met the following criteria: