Not available outside of the UK & Ireland.

Application

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The CpGenome™ Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice and enzymatically methylated at all CpGs by M.SssI methyltransferase. It can be used as a positive control for gene methylation studies, and is fully compatible with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847) and CpG WIZ™ methylation-specific amplification systems.

Materials Provided:

One vial containing 5 µg (20 µl) of CpGenome™ Universal Methylated Mouse DNA Standard at a concentration of 250 ng/µL.

Validation:

Methylation-specific PCR (MSP) was performed on the CpGenome™ Universal Methylated Mouse DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ™ Oct-4 kits (Catalog No. S7840) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for this Standard.

CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0

Storage and Stability

Recommended Storage: Stable at -20°C for up to 6 months from date of receipt.