alpha(2->3,6,8,9) Neuraminidase from Arthrobacter ureafaciens, recombinant, expressed in E. coli, buffered aqueous solution

Code: n8271-.2un D2-231

Not available outside of the UK & Ireland.

Biochem/physiol Actions

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.<...

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Not available outside of the UK & Ireland.

Biochem/physiol Actions

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.

Preparation Note

Expressed in glycosidase-free hosts.

Unit Definition

One unit will hydrolyze 1 µmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

foreign activity	β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
form	buffered aqueous solution
mol wt	95 kDa, 88 kDa
Quality Level	200
recombinant	expressed in E. coli
shipped in	wet ice
specific activity	≥135 units/mg protein
storage temp.	2-8°C

Cas Number

9001-67-6

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