Not available outside of the UK & Ireland.

Analysis Note

Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used: for phytohemagglutinin (PHA) swelling assayfor lymphocyte activation assay to evaluate cell-mediated immune response

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It has anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin is associated with toxic allergens and hemagglutinins. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.

General description

Lectins are carbohydrate-binding proteins, that contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Packaging

1, 5, 10 mg in glass bottle

Quality

Mitogenic activity at ≤10 µg per mL.