Not available outside of the UK & Ireland.

Analysis Note

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

Application

Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.

General description

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).