Not available outside of the UK & Ireland.

Application

The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

For Research Use Only. Not for use in diagnostic procedures.

Research CategoryCell Structure

The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

Components

ECM Array Plate: (PN: 90652) One 96-well plate with 12 strips. Each 8-well strip consists of 7 different human, ECM protein-coated wells (Collagen I, Collagen II, Collagen IV, Fibronectin, Laminin, Tenascin, Vitronectin) and one BSA-coated well (negative control). See the plate layout on data sheet.4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.CyQuant GR^™ Dye: (Part No. 90132) One vial - 75 µL.Assay Buffer: (Part No. 90601) One bottle - 100 mL.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Introduction:

Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON^® ECM Cell Adhesion Array Kit (Fluorometric) provides an ECM Array microtiter plate with a homogenous fluorescence detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion.

In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

⋅ Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)

⋅ Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)

⋅ Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)

⋅ Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM533)

⋅ Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM534)

⋅ Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorometric) (ECM535)

⋅ ECM Cell Adhesion Array Kit (Colorimetric) (ECM540)

⋅ QCM™ 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)

⋅ QCM™ 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)

⋅ QCM™ 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)

⋅ QCM™ 96-well Cell Invasion Assay (ECM555)

⋅ QCM™ 96-well Collagen-Based Cell Invasion Assay (ECM 556)

⋅ 24-well Insert Cell Migration and Invasion assay systems

⋅ CytoMatrix™ Cell Adhesion strips (ECM protein coated)

⋅ QuantiMatrix™ ECM protein ELISA kits



Test Principle:

The CHEMICON™ ECM Cell Adhesion Array kit utilizes a homogenous fluorescence detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are lysed and detected by the patented CyQuant GR™ dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3). Relative cell attachment is determined using a fluorescence plate reader.

Additional information about the ECM components provided in the kit:

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

GR is a trademark of Sigma-Aldrich Co. LLC

Packaging

1 plate in 96 wells

96 wells