Not available outside of the UK & Ireland.

Application

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.

Biochem/physiol Actions

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

Packaging

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

Physical form

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Unit Definition

One unit will release N-linked oligosaccharides from 1 µmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.